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Memorandum Immunophenotyping

Dear Client,
We often receive blood and bone marrow samples for immunophaenotyping without any clinical data. This makes analysis difficult and costly – with this information sheet we wish to explain why, and which data we require from you to ensure concise and cost-effective analysis of your specimens.

FACS analysis or immunophaenotyping of cells is a powerful method to detect and classify haematological neoplasms. It works by depicting defined antigens on the surface and in the cytoplasm of living cells with the help of monoclonal antibodies. It is therefore a time-consuming and costly method especially if you have to use large panels of antibodies to obtain a result.

Several hundreds of clustered antigens have been described which can be detected by thousands of antibodies and antibody combinations. This is why we need to know when the specimens arrives, which direction we should take for analysis, otherwise we would analyse hundreds of tests and still not find the correct diagnosis.

The most important directive is your clinical question or suspected diagnosis. Sometimes one might have several potential explanations for a given clinical problem. In these cases complementary basic information such as clinical history, clinical picture and results of other tests, especially morphology, help us to direct our search. Quite often, a given result from immunophaenotyping can only be interpreted in conjunction with morphology.

Please therefore ALWAYS send us the following:

2 ml of heparinized and EDTA-anticoagulated blood or bone marrow.
2 freshly made unstained slides of EDTA-anticoagulated blood or bone marrow (slides from heparinized blood cannot be interpreted).
It is imperative that you write clearly on the tube AND on the request form exactly what type of samples you are sending.
Kindly obtain all such samples on the day of shipment to us.
A short description of the clinical history and physical findings with A CLINICAL QUESTION (suspected diagnosis / differential diagnosis).
Blood count, differential, previous morphological findings.
Complementary information as applicable such as monoclonal peak, LDH, results of CT scans, etc.


Some of this information may not be available on the day you obtain the samples – in this case please ensure it is faxed or mailed to us whilst the sample is on its way to us and write on the data the patient’s barcode number so that we can match them together.

By adhering to the above, you will assist us in giving you a better and complete result – thank you.

Bioscientia Institut für Medizinische Diagnostik GmbH
Konrad-Adenauer-Strasse 17
55218 Ingelheim
Telephone: (49) 61 32 – 781 203/224/165
Telefax:      (49) 61 32 – 781 236


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